Gene Reporter Systems
Promega CHK1 Kinase Enzyme System
Recombinant full-length human CHK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CHK1 was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle.
Promega CSK Kinase Enzyme System
Recombinant full-length human CSK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CSK has been shown to downregulate the tyrosine kinase activity of c-src through tyrosine phosphorylation of the c-src carboxy terminus.
Promega PKCβ II Kinase Enzyme System
Recombinant full-length human PKC beta II was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC beta II is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
Promega Luciferase Control RNA
Luciferase Control RNA is an uncapped in vitro-transcribed RNA containing a 30-base poly(A) tail that produces functional luciferase when translated.
Promega ViviRen™ Live Cell Substrate
ViviRen Live Cell Substrate is a uniquely engineered coelenterazine that generates three- to fivefold brighter Renilla luciferase luminescence for super-sensitive quantitation of reporter gene, BRET and RNAi activity.
Promega Luciferase Assay Systems
An extremely sensitive reagent for rapid quantitation of firefly luciferase. Can be used for assays in single-tube luminometers or in multiwell-plate luminometers with auto-injectors.
Promega Calpain-Glo™ Assay System
The Calpain-Glo™ Protease Assay is a homogeneous, luminescent assay that measures calpain 1 (μ) and 2 (m) activities
Promega UDP-Glo Glycosyltransferase Assay + UDP-GlcNAc, 400 assays
The UDP-Glo Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega SYK Kinase Enzyme System
Recombinant full-length human SYK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SYK is a non-receptor protein tyrosine kinase that is widely expressed in hematopoietic cells.
Promega Intracellular TE Nano-Glo Substrate/Inhibitor, 10,000 assays
The Intracellular TE Nano-Glo Substrate/Inhibitor is a supplement for NanoBRET™ Target Engagement Assays.
Promega Ultra Pure UDP-GalNAc, 100mM, 50microl
UDP-sugar substrates are used with the UDP-Glo Glycosyltransferase Assay to detect the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega cAMP-Glo™ Max Assay System
The cAMP-Glo™ Max Assay is a homogeneous, bioluminescent and high-throughput assay to measure cyclic AMP (cAMP) levels in cells. Compounds that modulate GPCRs coupled with adenylate cyclase typically alter intracellular cAMP levels.
Promega ZAP70 Kinase Enzyme System
Recombinant full-length human ZAP70 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ZAP70 is a nonreceptor protein tyrosine kinase (part of the Syk/Zap70 family) that is involved in signaling by the T-cell antigen receptor.
Promega Ultra Pure UDP-GalNAc, 100mM, 250microl
UDP-sugar substrates are used with the UDP-Glo Glycosyltransferase Assay to detect the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega Ultra Pure UDP-Galactose, 100mM, 250microl
UDP-sugar substrates are used with the UDP-Glo Glycosyltransferase Assay to detect the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.