Gene Reporter Systems
Promega PKCι Kinase Enzyme System
Recombinant full-length human PKC iota was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein kinase C iota is a member of the protein kinase C family of serine-threonine kinases.
Promega JAK3 Kinase Enzyme System
Recombinant human JAK3 (amino acids 781-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. JAK3 is a member of the JAK family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction.
Promega Ultra Pure UDP-Glucose, 100mM, 250microl
UDP-sugar substrates are used with the UDP-Glo Glycosyltransferase Assay to detect the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega Proteasome-Glo™ 3 Assay
The Proteasome-Glo™ Cell-Based Assays are homogeneous, luminescent assays that measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in cultured cells.
Promega Kinase-Glo™ Max Luminescent Kinase Assay System
The Kinase-Glo™ Luminescent Kinase Assays are homogeneous non-radioactive systems for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction.
Promega LYN B Kinase Enzyme System
Recombinant full-length human LYN B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. LYN is a 56kDa tyrosine kinase that is similar to mouse T-lymphocyte-specific tyrosine kinase p56lck, v-yes, v-fgr and v-src.
Promega FLT1 Kinase Enzyme System
Recombinant human FLT1 (amino acids 784-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FLT1, also known as fms-like tyrosine kinase 1, is a member of the VEGFR family and is related to the oncogene ROS.
Promega Renilla-Glo™ Luciferase Assay System
The Renilla-Glo™ Luciferase Assay System is a single-addition reagent that generates a glow-type signal with Renilla luciferase.
Promega RSK2 Kinase Enzyme System
Recombinant full-length human RSK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RSK2 is a member of the RSK (ribosomal S6 kinase) family that are growth factor-regulated serine-threonine kinases.
Promega pBIND-GR Vector
NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.
Promega Luciferase Assay System Components
For use with Promega Luciferase Assay Systems (see cat. no. PRE1500)
Promega PKCζ Kinase Enzyme System
Recombinant full-length human PKC zeta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC zeta is an atypical isoform of the PKC family.
Promega CDK2/CyclinA2 Kinase Enzyme System
Recombinant full-length human CDK2 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed.
Promega Intracellular TE Nano-Glo Substrate/Inhibitor, 1,000 assays
The Intracellular TE Nano-Glo Substrate/Inhibitor is a supplement for NanoBRET™ Target Engagement Assays.
Promega Succinate-Glo JmjC Demethylase/Hydroxylase Assay, 1,000 assays
Succinate-Glo JmjC Demethylase/Hydroxylase Assay is a bioluminescent assay for detecting the activity of JumonjiC (JmjC) histone demethylases hydroxylases.